SNAP-Sec61B
(Plasmid #141152)

• Purpose: Fluorescent labeling of ER membrane in mammalian cells
• Depositing Lab: Gia Voeltz
• Publication: Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 10.1083/jcb.201911122.

Backbone

• Vector backbone: pAcGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4544
• Total vector size (bp): 4839
• Modifications to backbone: SNAP tag with a C-terminal linker was inserted into the NheI and XhoI sites of pAcGFP-C1.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sec61 beta
• Alt name: SEC61B
• Species: H. sapiens (human)
• Insert Size (bp): 295
• Mutation: L79M on Sec61B
• Entrez Gene: SEC61B
• Promoter: CMV
• Tag / Fusion Protein:
  • SNAP tag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: pFastBac Reverse

Resource Information

• Supplemental Documents:
  • SNAP-Sec61b.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry