CMV-loxp-DsRed-loxp-eGFP
(Plasmid #141148)

• Purpose: lentiviral expression vector to generate a hypoxia fate-mapping system
• Depositing Lab: Daniele Gilkes
• Publication: Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi: 10.1038/s41467-019-12412-1.

Backbone

• Vector backbone: pLenti CMV/TO Zeo DEST (644-1)
• Backbone manufacturer: Eric Campeau & Paul Kaufman (Addgene plasmid # 17294)
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Low salt LB media
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: loxp-DsRed-loxp-eGFP
• Alt name: Vector 1
• Species: Other

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • plentiloxpstoploxp.gb
• A portion of this plasmid was derived from a plasmid made by: loxp-DsRed-loxp-eGFP was PCR amplified from pMSCV-loxp-DsRed-loxp-eGFP-Puro-WPRE from Hans Clevers (Addgene plasmid # 32702)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

loxp-DsRed-loxp-eGFP was PCR amplified from pMSCV-loxp-DsRed-loxp-eGFP-Puro-WPRE (Addgene plasmid #32702) using primers containing Sal1 and Not1 restriction enzyme sites. The amplified PCR fragment was digested with Sal1 and Not1 and ligated into the pENTRA1 vector cut with Sal1 and Not1. The Gateway SystemTM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Zeo DEST (644-1) (#17294, Addgene) generating lentiviral vector 1.