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Addgene

4xHRE-MinTK-CRE-ODD
(Plasmid #141147)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRLSIN.cPPT.PGK-GFP.WPRE
  • Backbone manufacturer
    Didier Trono (Addgene plasmid # 12252)
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4xHRE-MinTK-CRE-ODD
  • Species
    Other
  • Mutation
    contains an altered Cre gene modified by the addition of an oxygen-dependent degradation domain (ODD)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4xHRE-MinTK-CRE-ODD was a gift from Daniele Gilkes (Addgene plasmid # 141147 ; http://n2t.net/addgene:141147 ; RRID:Addgene_141147)

  • For your References section:

    Fate-mapping post-hypoxic tumor cells reveals a ROS-resistant phenotype that promotes metastasis. Godet I, Shin YJ, Ju JA, Ye IC, Wang G, Gilkes DM. Nat Commun. 2019 Oct 24;10(1):4862. doi: 10.1038/s41467-019-12412-1. 10.1038/s41467-019-12412-1 PubMed 31649238
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