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Mammalian expression: Z-lock cofilin dark state mutant
(Plasmid #141136)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141136 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zdk2, Cofilin, LOV2
  • Species
    R. norvegicus (rat)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer universal cmv forward
  • 3′ sequencing primer universal SV40 poly(A) reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mammalian expression: Z-lock cofilin dark state mutant was a gift from Klaus Hahn (Addgene plasmid # 141136 ; http://n2t.net/addgene:141136 ; RRID:Addgene_141136)

  • For your References section:

    Optogenetic control of cofilin and alphaTAT in living cells using Z-lock. Stone OJ, Pankow N, Liu B, Sharma VP, Eddy RJ, Wang H, Putz AT, Teets FD, Kuhlman B, Condeelis JS, Hahn KM. Nat Chem Biol. 2019 Dec;15(12):1183-1190. doi: 10.1038/s41589-019-0405-4. Epub 2019 Nov 18. 10.1038/s41589-019-0405-4 PubMed 31740825
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