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E. coli expression: Z-lock cofilin dark state mutant
(Plasmid #141135)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141135 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zdk2, Cofilin, LOV2
  • Species
    R. norvegicus (rat)
  • Mutation
    D3 deletion, I32F in Zdk2

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer universal T7 forward
  • 3′ sequencing primer universal T7 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a point mutation in the Zdk portion of the dark control construct. In our hands, this had no effect on the experiments, and it is a position where it would not be expected to impact binding or optogenetic response.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E. coli expression: Z-lock cofilin dark state mutant was a gift from Klaus Hahn (Addgene plasmid # 141135 ; http://n2t.net/addgene:141135 ; RRID:Addgene_141135)

  • For your References section:

    Optogenetic control of cofilin and alphaTAT in living cells using Z-lock. Stone OJ, Pankow N, Liu B, Sharma VP, Eddy RJ, Wang H, Putz AT, Teets FD, Kuhlman B, Condeelis JS, Hahn KM. Nat Chem Biol. 2019 Dec;15(12):1183-1190. doi: 10.1038/s41589-019-0405-4. Epub 2019 Nov 18. 10.1038/s41589-019-0405-4 PubMed 31740825
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