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pMW-PahZ1 KT-1
(Plasmid #141134)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141134 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5709
  • Total vector size (bp) 6533
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PahZ1
  • Alt name
    Poly(aspartic acid) hydrolase-1 KT-1
  • Alt name
    PAAH-1
  • Species
    Sphingomonas Sp. KT-1
  • Insert Size (bp)
    825
  • Mutation
    Not the full-length gene. PahZ1 fragment starts at residue 41
  • GenBank ID
    BAC78155.1
  • Tag / Fusion Protein
    • 6x His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 Promoter Primer
  • 3′ sequencing primer T7 Terminator Primer
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Genscript

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see doi 10.1021/acssuschemeng.0c01158 for more detail about how this plasmid was created.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMW-PahZ1 KT-1 was a gift from Mitch Weiland (Addgene plasmid # 141134 ; http://n2t.net/addgene:141134 ; RRID:Addgene_141134)

  • For your References section:

    Gel Assay to Introduce Polymer Biodegradation in the Undergraduate Laboratory. Bolay A, Hiester W, Davis N, Weiland M. Journal of Chemical Education 10.1021/acs.jchemed.9b01204
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