pEGFP NFAT5 DBD DM
(Plasmid
#14113)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEGFP
- Backbone size w/o insert (bp) 4700
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNFAT5
-
SpeciesH. sapiens (human)
-
Insert Size (bp)900
-
MutationDNA binding domain aa 175 to 471, inactive allele
-
Entrez GeneNFAT5 (a.k.a. NF-AT5, NFATL1, NFATZ, OREBP, TONEBP)
-
Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Control inactive NFAT5 DBD mutant (DBD5). Also called DIM1.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEGFP NFAT5 DBD DM was a gift from Anjana Rao (Addgene plasmid # 14113 ; http://n2t.net/addgene:14113 ; RRID:Addgene_14113) -
For your References section:
The role of NFAT transcription factors in integrin-mediated carcinoma invasion. Jauliac S, Lopez-Rodriguez C, Shaw LM, Brown LF, Rao A, Toker A. Nat Cell Biol. 2002 Jul . 4(7):540-4. 10.1038/ncb816 PubMed 12080349