C331
(Plasmid
#141123)
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PurposeRhlI expressing plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 141123 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep15A
- Backbone size w/o insert (bp) 2735
- Total vector size (bp) 3338
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRhlI
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Insert Size (bp)603
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Entrez GenerhlI (a.k.a. PA3476)
- Promoter engineered lac promoter
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Tag
/ Fusion Protein
- ssrA degradation tag (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AGTGCGCTCGAGCTTCCC
- 3′ sequencing primer GATCAGTTGGAAGAATTTGTCCACT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C331 was a gift from Matthew Bennett (Addgene plasmid # 141123 ; http://n2t.net/addgene:141123 ; RRID:Addgene_141123) -
For your References section:
Majority sensing in synthetic microbial consortia. Alnahhas RN, Sadeghpour M, Chen Y, Frey AA, Ott W, Josic K, Bennett MR. Nat Commun. 2020 Jul 21;11(1):3659. doi: 10.1038/s41467-020-17475-z. 10.1038/s41467-020-17475-z PubMed 32694598