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Addgene

Oct4deltaPE-53BP1mCherry
(Plasmid #141122)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141122 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    mouse deltaPE Oct4-GFP transgenic reporter (addgene plasmid #52382)
  • Total vector size (bp) 22128
  • Modifications to backbone
    removed eGFP and added a PacI restriction site
  • Vector type
    Mouse Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Fragment of human 53BP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Entrez Gene
    TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site FseI (not destroyed)
  • 5′ sequencing primer ttaattaaCCACCATGGTGAGCAAG
  • 3′ sequencing primer ggccggccCTCATTCACCGGTGTTG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #52382 (with the modifications described) was used as the backbone. The insert sequence was cloned from addgene plasmid #19835

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Oct4deltaPE-53BP1mCherry was a gift from John Schimenti (Addgene plasmid # 141122 ; http://n2t.net/addgene:141122 ; RRID:Addgene_141122)
  • For your References section:

    A reporter mouse for in vivo detection of DNA damage in embryonic germ cells. Bloom JC, Schimenti JC. Genesis. 2020 Aug;58(8):e23368. doi: 10.1002/dvg.23368. Epub 2020 Apr 28. 10.1002/dvg.23368 PubMed 32343484