Oct4deltaPE-53BP1mCherry
(Plasmid
#141122)
-
PurposeConstruct used to generate a transgenic mouse strain with expression of 53BP1-mCherry in embryonic germ cells
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 141122 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonemouse deltaPE Oct4-GFP transgenic reporter (addgene plasmid #52382)
- Total vector size (bp) 22128
-
Modifications to backboneremoved eGFP and added a PacI restriction site
-
Vector typeMouse Targeting
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameFragment of human 53BP1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2200
-
Entrez GeneTP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
-
Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site FseI (not destroyed)
- 5′ sequencing primer ttaattaaCCACCATGGTGAGCAAG
- 3′ sequencing primer ggccggccCTCATTCACCGGTGTTG (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byAddgene plasmid #52382 (with the modifications described) was used as the backbone. The insert sequence was cloned from addgene plasmid #19835
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.01.29.925487v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Oct4deltaPE-53BP1mCherry was a gift from John Schimenti (Addgene plasmid # 141122 ; http://n2t.net/addgene:141122 ; RRID:Addgene_141122) -
For your References section:
A reporter mouse for in vivo detection of DNA damage in embryonic germ cells. Bloom JC, Schimenti JC. Genesis. 2020 Aug;58(8):e23368. doi: 10.1002/dvg.23368. Epub 2020 Apr 28. 10.1002/dvg.23368 PubMed 32343484