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pBS-Nvec-codonopti-Cas9-2xNLS
(Plasmid #141108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141108 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II SK(+)
  • Backbone manufacturer
    Genescript
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 7800
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nematostella codon optimized Cas9
  • Species
    Synthetic
  • Insert Size (bp)
    4500
  • Mutation
    All amino acids coded for by codons optimized for expression in Nematostella vectensis
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-Nvec-codonopti-Cas9-2xNLS was a gift from Matthew Gibson (Addgene plasmid # 141108 ; http://n2t.net/addgene:141108 ; RRID:Addgene_141108)

  • For your References section:

    Manipulation of Gene Activity in the Regenerative Model Sea Anemone, Nematostella vectensis. Hill EM, Chen CY, Del Viso F, Ellington LR, He S, Karabulut A, Paulson A, Gibson MC. Methods Mol Biol. 2022;2450:437-465. doi: 10.1007/978-1-0716-2172-1_23. 10.1007/978-1-0716-2172-1_23 PubMed 35359322
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