pUC19-phzAG
(Plasmid
#141106)
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PurposeContains a seven gene phzABCDEFG operon BioBrick
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 141106 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2641
- Total vector size (bp) 8976
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namephzABCDEFG
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SpeciesPseudomonas aeruginosa
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Insert Size (bp)6335
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19-phzAG was a gift from Martin Buck (Addgene plasmid # 141106 ; http://n2t.net/addgene:141106 ; RRID:Addgene_141106) -
For your References section:
Phenazines as model low-midpoint potential electron shuttles for photosynthetic bioelectrochemical systems. Clifford E, Bradley R, Wey L, Lawrence J, Chen X, Howe C and Zhang JZ.. Chem. Sci. (2021) 10.1039/D0SC05655C