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Addgene

pSB3C5-phzSM
(Plasmid #141105)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141105 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB3C5
  • Backbone size w/o insert (bp) 2695
  • Total vector size (bp) 5042
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phzSM
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    2347

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer TGCCACCTGACGTCTAAGAA
  • 3′ sequencing primer AGCCCCAATGATAACCCCAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSB3C5-phzSM was a gift from Martin Buck (Addgene plasmid # 141105 ; http://n2t.net/addgene:141105 ; RRID:Addgene_141105)

  • For your References section:

    Phenazines as model low-midpoint potential electron shuttles for photosynthetic bioelectrochemical systems. Clifford E, Bradley R, Wey L, Lawrence J, Chen X, Howe C and Zhang JZ.. Chem. Sci. (2021) 10.1039/D0SC05655C
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