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Purpose(Empty Backbone) Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 141104 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneentiSAM v2 (Puro) addgene#92062
- Backbone size (bp) 15524
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Modifications to backboneThe gRNA cloning site was replaced with the one from lentiCRISPR v2 (addgene# 52961)
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLentiV2-dCas9-VP64 was a gift from Igor Ulitsky (Addgene plasmid # 141104 ; http://n2t.net/addgene:141104 ; RRID:Addgene_141104)
Map uploaded by the depositor.
