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Addgene

SEPLuc
(Plasmid #141102)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141102 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-EF1-MCS-T2A-Puro
  • Backbone manufacturer
    System Biosciences (SBI)
  • Backbone size w/o insert (bp) 7068
  • Total vector size (bp) 8502
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SEP-Nanoluc
  • Alt name
    SEPLuc
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    1434
  • Promoter EF1α

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer EF1 Forward (CTCCACGCTTTGCCTGACCCTGCTT)
  • 3′ sequencing primer T2A Reverse (CGTCACCGCATGTTAGAAGACTTCCTCTGC)
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SEPLuc was a gift from Jeak Ling Ding (Addgene plasmid # 141102 ; http://n2t.net/addgene:141102 ; RRID:Addgene_141102)

  • For your References section:

    pHLuc, a Ratiometric Luminescent Reporter for in vivo Monitoring of Tumor Acidosis. Ong TT, Ang Z, Verma R, Koean R, Tam JKC, Ding JL. Front Bioeng Biotechnol. 2020 May 8;8:412. doi: 10.3389/fbioe.2020.00412. eCollection 2020. 10.3389/fbioe.2020.00412 PubMed 32457886
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