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pCS2+-H2B-pr-mEosFP
(Plasmid #141101)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141101 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone manufacturer
    RZPD
  • Backbone size w/o insert (bp) 4144
  • Total vector size (bp) 5221
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Histone 2B
  • Alt name
    H2B
  • Species
    H. sapiens (human), M. musculus (mouse), R. norvegicus (rat), D. rerio (zebrafish)
  • Promoter simian CMV IE94
  • Tag / Fusion Protein
    • pr-mEosFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site SnaBI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+-H2B-pr-mEosFP was a gift from Periklis Pantazis (Addgene plasmid # 141101 ; http://n2t.net/addgene:141101 ; RRID:Addgene_141101)

  • For your References section:

    Primed Track: Reliable Volumetric Single-cell Tracking and Lineage Tracing of Living Specimen with Dual-labeling Approaches. Welling M, Kalyviotis K, Pantazis P. Bio Protoc. 2020 Jun 5;10(11):e3645. doi: 10.21769/BioProtoc.3645. eCollection 2020 Jun 5. 10.21769/BioProtoc.3645 PubMed 33659315
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