fru-T2A-CsChrimson-tdTomato HDR plasmid
(Plasmid #141100)

• Purpose: Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene
• Depositing Lab: Leslie Vosshall
• Publication: Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10.7554/eLife.63982.

Backbone

• Vector backbone: pUC57
• Vector type: donor template

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
• Species: Synthetic; Chlamydomonas noctigama, Chloromonas subdivisa, Discosoma sp., Aequorea victoria, Aedes aegypti
• Insert Size (bp): 4635

Resource Information

• Supplemental Documents:
  • Fru-T2A-CsChrimson-TdT.gbk
• A portion of this plasmid was derived from a plasmid made by: CsChrimson-tdTomato was obtained from Vivek Jayaraman (Addgene plasmid #111544)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry