fru-T2A-CsChrimson-tdTomato HDR plasmid
(Plasmid
#141100)
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PurposePlasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 141100 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
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Vector typedonor template
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namefru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
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SpeciesSynthetic; Chlamydomonas noctigama, Chloromonas subdivisa, Discosoma sp., Aequorea victoria, Aedes aegypti
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Insert Size (bp)4635
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byCsChrimson-tdTomato was obtained from Vivek Jayaraman (Addgene plasmid #111544)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
fru-T2A-CsChrimson-tdTomato HDR plasmid was a gift from Leslie Vosshall (Addgene plasmid # 141100 ; http://n2t.net/addgene:141100 ; RRID:Addgene_141100) -
For your References section:
Fruitless mutant male mosquitoes gain attraction to human odor. Basrur NS, De Obaldia ME, Morita T, Herre M, von Heynitz RK, Tsitohay YN, Vosshall LB. Elife. 2020 Dec 7;9. pii: 63982. doi: 10.7554/eLife.63982. 10.7554/eLife.63982 PubMed 33284111