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Purpose(Empty Backbone) E. coli-Lactobacilli shuttle vector for recombineering template cloning
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 141096 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonecolE1, pAM beta
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCB591 was a gift from Chase Beisel (Addgene plasmid # 141096 ; http://n2t.net/addgene:141096 ; RRID:Addgene_141096) -
For your References section:
Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed That Editing Outcomes Can Vary Across Strains and Between Methods. Leenay RT, Vento JM, Shah M, Martino ME, Leulier F, Beisel CL. Biotechnol J. 2019 Mar;14(3):e1700583. doi: 10.1002/biot.201700583. Epub 2018 Sep 20. 10.1002/biot.201700583 PubMed 30156038