pCB578
(Plasmid #141095)

• Purpose: E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array
• Depositing Lab: Chase Beisel
• Publication: Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. doi: 10.1002/biot.201700583. Epub 2018 Sep 20.

Backbone

• Vector backbone: colE1, pAM beta
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Erythromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Cas9 and tracrRNA
• Species: S. pyogenes
• Insert Size (bp): 4650

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TAATTATTAGGGGGAGAAGG
• 3′ sequencing primer: TTTCGTTCATCCATAGTTGC

Gene/Insert 2

• Gene/Insert name: repeat-spacer-repeat array
• Insert Size (bp): 302

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTGTAATTTGTTTAATTGCCATTTCAATT
• 3′ sequencing primer: GGAACTACAAAATAAATTATAAGGAGGC

Resource Information

• Supplemental Documents:
  • pCB578 - 3545_Cas9RSR.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry