Brp-T2A-QF2w-Hsp70-3XP3-dsRed
(Plasmid
#141094)
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PurposeTemplate plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 141094 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepsL1180
- Backbone size w/o insert (bp) 3422
- Total vector size (bp) 8270
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Vector typeInsect Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namebruchpilot
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Alt nameBrp
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SpeciesAedes aegypti
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Insert Size (bp)136
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Mutationcoding sequence downstream of CRISPR cut site, synonymous codon substituted
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GenBank IDNCBI LOC5570381
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Brp-T2A-QF2w-Hsp70-3XP3-dsRed was a gift from Carolyn McBride (Addgene plasmid # 141094 ; http://n2t.net/addgene:141094 ; RRID:Addgene_141094) -
For your References section:
Development of a pan-neuronal genetic driver in Aedes aegypti mosquitoes. Zhao Z, Tian D, McBride CS. Cell Rep Methods. 2021 Jul 26;1(3):100042. doi: 10.1016/j.crmeth.2021.100042. Epub 2021 Jun 30. 10.1016/j.crmeth.2021.100042 PubMed 34590074
Map uploaded by the depositor.
