pYPQ166-OsPE2
(Plasmid #141080)

• Purpose: Gateway entry clone for CRISPR-Cas9(H840A) prime editing
• Depositing Lab: Yiping Qi
• Publication: Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(20)30072-1. doi: 10.1016/j.molp.2020.03.010.

Backbone

• Vector backbone: pYPQ165
• Total vector size (bp): 9968
• Vector type: Plant Expression, CRISPR ; Gateway compatible zCas9(H840A)-OsM-MLV-RT

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: zCas9(H840A)-OsM-MLV-RT
• Tags / Fusion Proteins:
  • 3x FLAG, NLS (N terminal on insert)
  • NLS (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pYPQ166-OsPE2.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Protein sequence of engineered M-MLV reverse transcriptase (D200N, L603W, T306K, W313F, T330P) was obtained from published work: Anzalone, A.V., Randolph, P.B., Davis, J.R., Sousa, A.A., Koblan, L.W., Levy, J.M., Chen, P.J., Wilson, C., Newby, G.A., Raguram, A. and Liu, D.R., 2019. Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), pp.149-157.