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Addgene

pQE-30-RexYFP
(Plasmid #141070)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 141070 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE30
  • Backbone size w/o insert (bp) 3425
  • Total vector size (bp) 4814
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RexYFP
  • Species
    Synthetic
  • Insert Size (bp)
    1389
  • Mutation
    changed Leucine 169 to Proline, changed Tyrosine 175 to Asparagine, changed Aspartic Acid 313 to Glycine
  • Promoter T5
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GCT TTG TGA GCG GAT AAC A
  • 3′ sequencing primer CCG AGC GTT CTG AAC AAA TC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE-30-RexYFP was a gift from Vsevolod Belousov (Addgene plasmid # 141070 ; http://n2t.net/addgene:141070 ; RRID:Addgene_141070)

  • For your References section:

    Genetically encoded fluorescent indicator for imaging NAD/NADH ratio changes in different cellular compartments. Bilan DS, Matlashov ME, Gorokhovatsky AY, Schultz C, Enikolopov G, Belousov VV. Biochim Biophys Acta. 2013 Nov 25. pii: S0304-4165(13)00519-9. doi: 10.1016/j.bbagen.2013.11.018. 10.1016/j.bbagen.2013.11.018 PubMed 24286672
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