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Addgene

pDESTFLAGHApuro3-Ago2Q545AK570A
(Plasmid #14105)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14105 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDESTFLAGHApuro3
  • Backbone size w/o insert (bp) 5368
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ago2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2562
  • Mutation
    changed Gln545 to Ala and Lys570 to Ala
  • Entrez Gene
    AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
  • Tag / Fusion Protein
    • FLAG/HA (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site gateway (not destroyed)
  • 3′ cloning site gateway (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDESTFLAGHApuro3-Ago2Q545AK570A was a gift from Thomas Tuschl (Addgene plasmid # 14105 ; http://n2t.net/addgene:14105 ; RRID:Addgene_14105)

  • For your References section:

    Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi protein. Ma JB, Yuan YR, Meister G, Pei Y, Tuschl T, Patel DJ. Nature. 2005 Mar 31. 434(7033):666-70. 10.1038/nature03514 PubMed 15800629
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