pDESTFLAGHApuro3-Ago2K533AK570A
(Plasmid
#14104)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14104 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDESTFLAGHApuro3
- Backbone size w/o insert (bp) 5368
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAgo2
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SpeciesH. sapiens (human)
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Insert Size (bp)2562
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Mutationchanged Lys533 to Ala, Lys570 to Alanine
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Entrez GeneAGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)
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Tag
/ Fusion Protein
- FLAG/HA (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site gateway (not destroyed)
- 3′ cloning site gateway (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDESTFLAGHApuro3-Ago2K533AK570A was a gift from Thomas Tuschl (Addgene plasmid # 14104 ; http://n2t.net/addgene:14104 ; RRID:Addgene_14104) -
For your References section:
Structural basis for 5'-end-specific recognition of guide RNA by the A. fulgidus Piwi protein. Ma JB, Yuan YR, Meister G, Pei Y, Tuschl T, Patel DJ. Nature. 2005 Mar 31. 434(7033):666-70. 10.1038/nature03514 PubMed 15800629