pUC-DEST-cjSTELLA-mCerulean-PuroTK
(Plasmid
#141029)
-
PurposeReporter integration
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 141029 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepUC
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemCerulean
-
SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains a single G nucleotide deletion in attB1. This deletion is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pUC-DEST-cjSTELLA-mCerulean-PuroTK was a gift from Hideyuki Okano (Addgene plasmid # 141029 ; http://n2t.net/addgene:141029 ; RRID:Addgene_141029) -
For your References section:
A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology. Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, Nakamura M, Sasaki E, Shiozawa S, Okano H. PLoS One. 2019 Aug 27;14(8):e0221164. doi: 10.1371/journal.pone.0221164. eCollection 2019. 10.1371/journal.pone.0221164 PubMed 31454364