Myr-(iSH2-p85)-p110alpha-Myc
(Plasmid #1410)

• Depositing Lab: Lewis Cantley
• Publication: pBS-p85alpha (unpublished)

Backbone

• Vector backbone: pSRalpha
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha)
• Alt name: p85 alpha
• Alt name: p110 alpha
• Alt name: Pik3r1, Pik3ca
• Species: M. musculus (mouse)
• Insert Size (bp): 2000
• Entrez Gene: Pik3ca (a.k.a. 6330412C24Rik, caPI3K, p110, p110alpha)
• Entrez Gene: Pik3r1 (a.k.a. PI3K, p50alpha, p55alpha, p85alpha)
• Promoter: SRalpha promoter
• Tag / Fusion Protein:
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: SV40pro-F2 (5'-CCCCATGGCTGACTAATTTTT-3')
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that NcoI is not a unique enzyme and will cut the plasmid DNA at five locations.