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pCR-BluntII-TOPO-RhoC
(Plasmid #14099)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14099 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCR-BluntII-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3519
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Human RhoC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    645
  • Entrez Gene
    RHOC (a.k.a. ARH9, ARHC, H9, RHOH9)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR-BluntII-TOPO-RhoC was a gift from Richard Hynes (Addgene plasmid # 14099 ; http://n2t.net/addgene:14099 ; RRID:Addgene_14099)

  • For your References section:

    Genomic analysis of metastasis reveals an essential role for RhoC. Clark EA, Golub TR, Lander ES, Hynes RO. Nature. 2000 Aug 3. 406(6795):532-5. 10.1038/35020106 PubMed 10952316
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