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Addgene

pMA4.1 (mouse integrin alpha 4 probe)
(Plasmid #14098)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14098 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript II SK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2950
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mouse Alpha 4 Integrin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1000
  • GenBank ID
    X53176
  • Entrez Gene
    Itga4 (a.k.a. CD49D, Itga4B)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMA4.1 (mouse integrin alpha 4 probe) was a gift from Richard Hynes (Addgene plasmid # 14098 ; http://n2t.net/addgene:14098 ; RRID:Addgene_14098)
  • For your References section:

    Cell adhesion events mediated by alpha 4 integrins are essential in placental and cardiac development. Yang JT, Rayburn H, Hynes RO. Development. 1995 Feb . 121(2):549-60. PubMed 7539359