pMA4.1 (mouse integrin alpha 4 probe)
(Plasmid
#14098)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14098 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II SK+
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2950
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMouse Alpha 4 Integrin
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SpeciesM. musculus (mouse)
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Insert Size (bp)1000
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GenBank IDX53176
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Entrez GeneItga4 (a.k.a. CD49D, Itga4B)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMA4.1 (mouse integrin alpha 4 probe) was a gift from Richard Hynes (Addgene plasmid # 14098 ; http://n2t.net/addgene:14098 ; RRID:Addgene_14098) -
For your References section:
Cell adhesion events mediated by alpha 4 integrins are essential in placental and cardiac development. Yang JT, Rayburn H, Hynes RO. Development. 1995 Feb . 121(2):549-60. PubMed 7539359