pMA5.1 (mouse integrin alpha 5 probe)
(Plasmid
#14097)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14097 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II SK+
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2950
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMouse Alpha 5 Integrin
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SpeciesM. musculus (mouse)
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Insert Size (bp)860
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GenBank IDNM_010577
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Entrez GeneItga5 (a.k.a. Cd49e, Fnra, VLA5)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMA5.1 (mouse integrin alpha 5 probe) was a gift from Richard Hynes (Addgene plasmid # 14097 ; http://n2t.net/addgene:14097 ; RRID:Addgene_14097) -
For your References section:
Embryonic mesodermal defects in alpha 5 integrin-deficient mice. Yang JT, Rayburn H, Hynes RO. Development. 1993 Dec . 119(4):1093-105. PubMed 7508365
Map uploaded by the depositor.