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Addgene

pMA5.1 (mouse integrin alpha 5 probe)
(Plasmid #14097)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14097 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript II SK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2950
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mouse Alpha 5 Integrin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    860
  • GenBank ID
    NM_010577
  • Entrez Gene
    Itga5 (a.k.a. Cd49e, Fnra, VLA5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMA5.1 (mouse integrin alpha 5 probe) was a gift from Richard Hynes (Addgene plasmid # 14097 ; http://n2t.net/addgene:14097 ; RRID:Addgene_14097)
  • For your References section:

    Embryonic mesodermal defects in alpha 5 integrin-deficient mice. Yang JT, Rayburn H, Hynes RO. Development. 1993 Dec . 119(4):1093-105. PubMed 7508365