pWZL blast HA goosecoid
(Plasmid #14096)

• Depositing Lab: Bob Weinberg
• Publication: Hartwell et al Proc Natl Acad Sci U S A. 2006 Dec 12. 103(50):18969-74.

Backbone

• Vector backbone: pWZL Blast
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: goosecoid
• Species: M. musculus (mouse)
• Insert Size (bp): 800
• Entrez Gene: Gsc
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: pBABE 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Martin Blum

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry