pMbd3EosHaloTAF_Hyg
(Plasmid
#140958)
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PurposeTargeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140958 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II KS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 9674
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Vector typeMouse Targeting
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemethyl-CpG binding protein 3
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SpeciesM. musculus (mouse), Synthetic
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Insert Size (bp)6733
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GenBank IDNM_001281453
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Entrez GeneMbd3
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Tags
/ Fusion Proteins
- Eos (C terminal on insert)
- Halo (C terminal on insert)
- TEV (C terminal on insert)
- Avi (C terminal on insert)
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMbd3EosHaloTAF_Hyg was a gift from Brian Hendrich (Addgene plasmid # 140958 ; http://n2t.net/addgene:140958 ; RRID:Addgene_140958)