pHB-3
(Plasmid
#140956)
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PurposeFor PHB production in E. coli (T7-pAC). Please note that this is listed as #140955 in the publication.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140956 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHB-2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namephaCAB
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Alt namepolyhydroxybutyrate operon (from Ralstonia eutropha H16 chromosome 1)
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SpeciesRalstonia eutropha H16
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GenBank IDAM260479.1, bases 1,557,353 to. 1,561,203
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHB-3 was a gift from Kang Zhou (Addgene plasmid # 140956 ; http://n2t.net/addgene:140956 ; RRID:Addgene_140956) -
For your References section:
Using biopolymer bodies for encapsulation of hydrophobic products in bacterium. Liu Y, Low ZJ, Ma X, Liang H, Sinskey AJ, Stephanopoulos G, Zhou K. Metab Eng. 2020 Sep;61:206-214. doi: 10.1016/j.ymben.2020.04.006. Epub 2020 Apr 24. 10.1016/j.ymben.2020.04.006 PubMed 32339760