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Addgene

pHB-2
(Plasmid #140955)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140955 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHB-2
  • Modifications to backbone
    random deletion/mutation within LacI_T7 region
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    phaCAB
  • Alt name
    polyhydroxybutyrate operon (from Ralstonia eutropha H16 chromosome 1)
  • Species
    Ralstonia eutropha H16
  • Mutation
    R238S mutation in PhaC
  • GenBank ID
    AM260479.1, bases 1,557,353 to. 1,561,203

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHB-2 was a gift from Kang Zhou (Addgene plasmid # 140955 ; http://n2t.net/addgene:140955 ; RRID:Addgene_140955)

  • For your References section:

    Using biopolymer bodies for encapsulation of hydrophobic products in bacterium. Liu Y, Low ZJ, Ma X, Liang H, Sinskey AJ, Stephanopoulos G, Zhou K. Metab Eng. 2020 Sep;61:206-214. doi: 10.1016/j.ymben.2020.04.006. Epub 2020 Apr 24. 10.1016/j.ymben.2020.04.006 PubMed 32339760
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