Gr4-T2A-QF2 HDR plasmid
(Plasmid
#140944)
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PurposePlasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140944 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepuc19
- Total vector size (bp) 7630
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
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SpeciesSynthetic; Aedes aegpyti
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Insert Size (bp)4950
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byQF2 was obtained from Chris Potter, Addgene plasmid #104876
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Gr4-T2A-QF2 HDR plasmid was a gift from Leslie Vosshall (Addgene plasmid # 140944 ; http://n2t.net/addgene:140944 ; RRID:Addgene_140944) -
For your References section:
Sensory Discrimination of Blood and Floral Nectar by Aedes aegypti Mosquitoes. Jove V, Gong Z, Hol FJH, Zhao Z, Sorrells TR, Carroll TS, Prakash M, McBride CS, Vosshall LB. Neuron. 2020 Dec 23;108(6):1163-1180.e12. doi: 10.1016/j.neuron.2020.09.019. Epub 2020 Oct 12. 10.1016/j.neuron.2020.09.019 PubMed 33049200
Map uploaded by the depositor.
