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Addgene

Ir7f-T2A-QF2 HDR plasmid
(Plasmid #140942)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140942 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    puc19
  • Total vector size (bp) 7249

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ir7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
  • Species
    Synthetic; Aedes aegpyti
  • Insert Size (bp)
    4569
  • Mutation
    4 point mutations (annotated on plasmid map) were introduced into Ir7f sequence upstream of guide cut site

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    QF2 was obtained from Chris Potter, Addgene plasmid #104876

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ir7f-T2A-QF2 HDR plasmid was a gift from Leslie Vosshall (Addgene plasmid # 140942 ; http://n2t.net/addgene:140942 ; RRID:Addgene_140942)

  • For your References section:

    Sensory Discrimination of Blood and Floral Nectar by Aedes aegypti Mosquitoes. Jove V, Gong Z, Hol FJH, Zhao Z, Sorrells TR, Carroll TS, Prakash M, McBride CS, Vosshall LB. Neuron. 2020 Dec 23;108(6):1163-1180.e12. doi: 10.1016/j.neuron.2020.09.019. Epub 2020 Oct 12. 10.1016/j.neuron.2020.09.019 PubMed 33049200
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