pLenti-lox-GFP shRNA p19-2
(Plasmid #14091)

• Depositing Lab: Tyler Jacks
• Publication: Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

Backbone

• Vector backbone: pLL3.7
• Backbone manufacturer: Available from Addgene (#11795)
• Backbone size w/o insert (bp): 8000
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p19 ARF shRNA -2
• Alt name: p19ARF
• Alt name: ARF-INK4a
• Species: M. musculus (mouse)
• Insert Size (bp): 50
• Entrez Gene: Cdkn2a (a.k.a. ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19<ARF>, p19ARF)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: H1

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

p19 ARF shRNA expressed from a lentiviral vector. The p19 ARF shRNA together with the H1 promoter was cloned from pSIRIP-puro into pLL3.7. Therefore, although the shRNA is normally expressed from the U6 promoter of pLL3.7, p19-ARF shRNA is expressed here from the H1 promoter.