pCX-SpiCee-NES
(Plasmid
#140901)
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Purposemammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140901 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCX
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpiCee-NES
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Alt nameCalcium Sponge-NES
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SpeciesSynthetic
- Promoter CAG
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Tag
/ Fusion Protein
- mRFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCX-SpiCee-NES was a gift from Xavier Nicol (Addgene plasmid # 140901 ; http://n2t.net/addgene:140901 ; RRID:Addgene_140901) -
For your References section:
SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation. Ros O, Baudet S, Zagar Y, Loulier K, Roche F, Couvet S, Aghaie A, Atkins M, Louail A, Petit C, Metin C, Mechulam Y, Nicol X. Cell Rep. 2020 Jul 21;32(3):107934. doi: 10.1016/j.celrep.2020.107934. 10.1016/j.celrep.2020.107934 PubMed 32697983
Map uploaded by the depositor.
