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Addgene

pCR2.1-p55alpha
(Plasmid #1409)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1409 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1
  • Backbone size w/o insert (bp) 3900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PI3K regulatory subunit p55alpha
  • Alt name
    Pik3r1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1400
  • Entrez Gene
    Pik3r1 (a.k.a. PI3K, p50alpha, p55alpha, p85alpha)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Fumi Kanai

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

does not express, only to subclone into other plasmid, contains internal BglII site

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR2.1-p55alpha was a gift from Lewis Cantley (Addgene plasmid # 1409 ; http://n2t.net/addgene:1409 ; RRID:Addgene_1409)
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