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pET11-His6-Pif6-sfGFP-ssrA
(Plasmid #140891)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140891 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET11a
  • Backbone manufacturer
    Novagen
  • Total vector size (bp) 6751
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PIF-sfGFP-ssrA
  • Species
    Synthetic
  • Insert Size (bp)
    1110
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer cggtgatgtcggcgatatag
  • 3′ sequencing primer tgctagttattgctcagcgg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET11-His6-Pif6-sfGFP-ssrA was a gift from Neal Devaraj (Addgene plasmid # 140891 ; http://n2t.net/addgene:140891 ; RRID:Addgene_140891)

  • For your References section:

    Lipid sponge droplets as programmable synthetic organelles. Bhattacharya A, Niederholtmeyer H, Podolsky KA, Bhattacharya R, Song JJ, Brea RJ, Tsai CH, Sinha SK, Devaraj NK. Proc Natl Acad Sci U S A. 2020 Aug 4;117(31):18206-18215. doi: 10.1073/pnas.2004408117. Epub 2020 Jul 21. 10.1073/pnas.2004408117 PubMed 32694212
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