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Addgene

pSIRIP-puro shRNA p19-1
(Plasmid #14089)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14089 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSIRIP-puro
  • Backbone manufacturer
    Todd Golub Lab
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p19 ARF shRNA - 1
  • Alt name
    p19ARF
  • Alt name
    ARF-INK4a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    50
  • Entrez Gene
    Cdkn2a (a.k.a. ARF-INK4a, Arf, INK4a-ARF, Ink4a/Arf, MTS1, Pctr1, p16, p16(INK4a), p16INK4a, p19<ARF>, p19ARF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer H1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

p19 ARF shRNA expressed from a self-inactivating retroviral vector. See author's map for information about the pSIRIP-puro vector backbone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIRIP-puro shRNA p19-1 was a gift from Tyler Jacks (Addgene plasmid # 14089 ; http://n2t.net/addgene:14089 ; RRID:Addgene_14089)

  • For your References section:

    Acute mutation of retinoblastoma gene function is sufficient for cell cycle re-entry. Sage J, Miller AL, Perez-Mancera PA, Wysocki JM, Jacks T. Nature. 2003 Jul 10. 424(6945):223-8. 10.1038/nature01764 PubMed 12853964
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