pBabe-puro largeTcDNA
(Plasmid #14088)

• Depositing Lab: William Hahn
• Publication: Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Backbone

• Vector backbone: pBabe-puro
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Large T antigen
• Alt name: Large T cDNA
• Alt name: pBNT
• Alt name: LTc
• Species: simian virus
• Insert Size (bp): 2200
• Entrez Gene: SV40gp6 (a.k.a. SV40gp6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pBabe 5'

Resource Information

• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry