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Addgene

pT7gRNA-ttn.1_C3
(Plasmid #140866)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140866 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7gRNA-ccdb (Addgene 140864)
  • Vector type
    In vitro transcription of sgRNAs

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zebrafish ttn.1_C3 sgRNA for in vitro transcription
  • Species
    D. rerio (zebrafish), Synthetic
  • Entrez Gene
    ttn.1 (a.k.a. TTN, hm:zeh1256r, im:6911926, im:7146708, si:dZ167C3.1, si:dz249n21.1, ttnb, ttnl, wu:fc09a12)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7gRNA-ttn.1_C3 was a gift from Rob Parton (Addgene plasmid # 140866 ; http://n2t.net/addgene:140866 ; RRID:Addgene_140866)

  • For your References section:

    In vivo cell biological screening identifies an endocytic capture mechanism for T-tubule formation. Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim YW, Parton RG. Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w. 10.1038/s41467-020-17486-w PubMed 32709891
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