pLz37_CB_operon_CsoS2_N1-N4
(Plasmid #140856)

• Purpose: Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.
• Depositing Lab: David Savage
• Publication: Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 10.1038/s41594-020-0387-7. Epub 2020 Mar 2.

Backbone

• Vector backbone: NS3
• Backbone size w/o insert (bp): 4891
• Total vector size (bp): 13243
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
• Species: Halothiobacillus neapolitanus
• Insert Size (bp): 8713

Resource Information

• Supplemental Documents:
  • plz37-phncb10_natives2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There exists a G534V mismatch in the carboxysome shell polypeptide compared to the reference sequence. The authors do not expect the mutation to significantly alter protein function but this should be functionally validated.