pBz172_CsoS2_N1_GFP
(Plasmid
#140852)
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PurposeHis-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140852 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-14b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4614
- Total vector size (bp) 5685
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Modifications to backboneelimination of built-in His-tag and thrombin recognition site; introduction of BsaI sites for Golden Gate assembly
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCsoS2 NTD repeat 1 fused to GFP
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Alt name[N1]-GFP
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Insert Size (bp)1077
- Promoter T7
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Tags
/ Fusion Proteins
- Hexahistidine tag (N terminal on insert)
- Superfolder GFP (C terminal on insert)
- Strep-Tag II (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBz172_CsoS2_N1_GFP was a gift from David Savage (Addgene plasmid # 140852 ; http://n2t.net/addgene:140852 ; RRID:Addgene_140852) -
For your References section:
Multivalent interactions between CsoS2 and Rubisco mediate alpha-carboxysome formation. Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage DF. Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 10.1038/s41594-020-0387-7. Epub 2020 Mar 2. 10.1038/s41594-020-0387-7 PubMed 32123388