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pLz55_polyPro_random_Npeptide
(Plasmid #140846)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140846 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-14b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4614
  • Total vector size (bp) 4952
  • Modifications to backbone
    elimination of built-in His-tag and thrombin recognition site; introduction of BsaI sites for Golden Gate assembly
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    polyproline helix with randomized CsoS2 N-peptide
  • Insert Size (bp)
    345
  • GenBank ID
  • Promoter T7
  • Tag / Fusion Protein
    • Hexahistidine tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLz55_polyPro_random_Npeptide was a gift from David Savage (Addgene plasmid # 140846 ; http://n2t.net/addgene:140846 ; RRID:Addgene_140846)

  • For your References section:

    Multivalent interactions between CsoS2 and Rubisco mediate alpha-carboxysome formation. Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage DF. Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 10.1038/s41594-020-0387-7. Epub 2020 Mar 2. 10.1038/s41594-020-0387-7 PubMed 32123388
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