pBz15_Strep_Rubisco
(Plasmid #140839)

• Purpose: wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone
• Depositing Lab: David Savage
• Publication: Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 10.1038/s41594-020-0387-7. Epub 2020 Mar 2.

Backbone

• Vector backbone: pET-14b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4614
• Total vector size (bp): 6426
• Modifications to backbone: elimination of built-in His-tag and thrombin recognition site; introduction of BsaI sites for Golden Gate assembly
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Form IA Rubisco; CbbL, CbbS
• Species: Halothiobacillus neapolitanus
• Insert Size (bp): 1818
• GenBank ID: AAC32549.1 AAC32550.1
• Promoter: T7
• Tag / Fusion Protein:
  • Strep II (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Resource Information

• Supplemental Documents:
  • pbz15038-ecolirbs-cbbl-cstrepii-cbbs.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry