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Addgene

pCX-SpiCee
(Plasmid #140836)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140836 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCX
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpiCee
  • Alt name
    Calcium Sponge
  • Species
    Synthetic
  • Promoter CAG
  • Tag / Fusion Protein
    • mRFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCX-SpiCee was a gift from Xavier Nicol (Addgene plasmid # 140836 ; http://n2t.net/addgene:140836 ; RRID:Addgene_140836)

  • For your References section:

    SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation. Ros O, Baudet S, Zagar Y, Loulier K, Roche F, Couvet S, Aghaie A, Atkins M, Louail A, Petit C, Metin C, Mechulam Y, Nicol X. Cell Rep. 2020 Jul 21;32(3):107934. doi: 10.1016/j.celrep.2020.107934. 10.1016/j.celrep.2020.107934 PubMed 32697983
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