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Addgene

pPBCAG-rtTAM2-IH
(Plasmid #140827)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140827 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pPB-MCSfw-pA
  • Total vector size (bp) 8113
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    rtTAM2
  • Insert Size (bp)
    747

Gene/Insert 2

  • Gene/Insert name
    HygR
  • Alt name
    hygromycin B phosphotransferase
  • Species
    Escherichia coli UMNF18
  • Insert Size (bp)
    1020

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBCAG-rtTAM2-IH was a gift from Hideyuki Okano (Addgene plasmid # 140827 ; http://n2t.net/addgene:140827 ; RRID:Addgene_140827)
  • For your References section:

    Naive-like ESRRB(+) iPSCs with the Capacity for Rapid Neural Differentiation. Kisa F, Shiozawa S, Oda K, Yoshimatsu S, Nakamura M, Koya I, Kawai K, Suzuki S, Okano H. Stem Cell Reports. 2017 Dec 12;9(6):1825-1838. doi: 10.1016/j.stemcr.2017.10.008. Epub 2017 Nov 9. 10.1016/j.stemcr.2017.10.008 PubMed 29129686