pPBCAG-rtTAM2-IH
(Plasmid
#140827)
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PurposeExpresses rtTAM2-IRES-HygR in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140827 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPB-MCSfw-pA
- Total vector size (bp) 8113
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namertTAM2
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Insert Size (bp)747
Gene/Insert 2
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Gene/Insert nameHygR
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Alt namehygromycin B phosphotransferase
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SpeciesEscherichia coli UMNF18
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Insert Size (bp)1020
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPBCAG-rtTAM2-IH was a gift from Hideyuki Okano (Addgene plasmid # 140827 ; http://n2t.net/addgene:140827 ; RRID:Addgene_140827) -
For your References section:
Naive-like ESRRB(+) iPSCs with the Capacity for Rapid Neural Differentiation. Kisa F, Shiozawa S, Oda K, Yoshimatsu S, Nakamura M, Koya I, Kawai K, Suzuki S, Okano H. Stem Cell Reports. 2017 Dec 12;9(6):1825-1838. doi: 10.1016/j.stemcr.2017.10.008. Epub 2017 Nov 9. 10.1016/j.stemcr.2017.10.008 PubMed 29129686