Skip to main content
Addgene

phlFd-mLin
(Plasmid #140793)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140793 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    FRT-Hygromycin
  • Total vector size (bp) 7528
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phlFd-mLinearizer
  • Alt name
    CMV-phlFO2-PhlFR-2A-mCherry
  • Insert Size (bp)
    3476
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CATCAAGTGTATCATATGCC
  • 3′ sequencing primer TGTTTGTCCACCACCGGTAGAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phlFd-mLin was a gift from Gabor Balazsi (Addgene plasmid # 140793 ; http://n2t.net/addgene:140793 ; RRID:Addgene_140793)

  • For your References section:

    Multiplexed Gene Expression Tuning with Orthogonal Synthetic Gene Circuits. Szenk M, Yim T, Balazsi G. ACS Synth Biol. 2020 Apr 17;9(4):930-939. doi: 10.1021/acssynbio.9b00534. Epub 2020 Mar 23. 10.1021/acssynbio.9b00534 PubMed 32167761
Related items:
Commonly requested with: