Sox17(2A-phiC31o)
(Plasmid #140762)

• Purpose: Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.
• Depositing Lab: Hideyuki Okano
• Publication: Serizawa et al Development. 2019 Nov 4;146(21). pii: dev.174938. doi: 10.1242/dev.174938.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 2396
• Total vector size (bp): 18841
• Vector type: Mouse Targeting
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: phiC31o
• Alt name: codon-optimized phiC31
• Species: Synthetic
• Insert Size (bp): 1839
• Promoter: Endogenous Sox17

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • Sox17(2A-phiC31o).gb
• A portion of this plasmid was derived from a plasmid made by: phiC31o was cloned from pPGKPhiC31obpA (a gift from Philippe Soriano; Addgene plasmid #13795; Raymond and Soriano, PLoS ONE 2, e162, 2007).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry