T/brachyury(2A-Dre)
(Plasmid #140761)

• Purpose: Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.
• Depositing Lab: Hideyuki Okano
• Publication: Serizawa et al Development. 2019 Nov 4;146(21). pii: dev.174938. doi: 10.1242/dev.174938.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 2396
• Total vector size (bp): 18841
• Vector type: Mouse Targeting
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dre
• Species: Synthetic
• Insert Size (bp): 1074
• Promoter: Endogenous T/brachyury

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • T:brachyury(2A-Dre).gb
• A portion of this plasmid was derived from a plasmid made by: Dre was cloned from pCAGGS-dre-ires-puro (a gift from A. Francis Stewart; Anastassiadis et al., Dis. Model. Mech. 2, 508-515, 2009).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry